منابع مشابه
Chemiluminescence immunoassay
The use of chemiluininescent molecules as non—isotopic alternatives to 125 in immunoassay permits the development of sensitive, stable assay systems.
متن کاملChemiluminescence immunoassay: an overview.
A large number of techniques cxist though some arc more suitable than others for the measurement of a particular compound, considerations being given to its size and its concentration. Howcver, all immunoassays require two components, firstly an antibody capable of specifically binding the compound and, secondly, a tracer which permits this binding reaction to be observed. In classical radioimm...
متن کاملDirect chemiluminescence immunoassay of estradiol in saliva.
A sensitive and simple direct solid-phase chemiluminescence immunoassay is described for estradiol in saliva. In this assay, a second antibody is bound to the wells of microtiter plates. Either buffer with standards or saliva (100 microL) is incubated in these wells with monoclonal anti-estradiol antibody and with estradiol-isoluminol conjugate. Incubation time is 2 h. Chemiluminescence of the ...
متن کاملChemiluminescence immunoassay of aldosterone in serum.
A chemiluminescence immunoassay (CLI) for the direct measurement of aldosterone in serum was developed with aminobutylethyl isoluminol (ABEI) as the label. In this competitive assay the samples are incubated with sample, antibody, aldosterone-carboxymethyl oxime-ABEI, and paramagnetic particles coated with second antibody. After magnetic separation and washing, the samples are incubated with 20...
متن کاملChemiluminescence immunoassay for somatomedin C in serum.
To select the best tracer for use in a competitive immunoassay, we conjugated human somatomedin C (SmC) to various chemiluminescent compounds via two different synthetic pathways. Naphthylhydrazides and arylhydrazides, used as the labels, were incorporated via their imidate or their succinimide esters. Conjugating the carboxy terminal of (amino ethyl)ethyl-isoluminol to SmC via a succinimide li...
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ژورنال
عنوان ژورنال: TrAC Trends in Analytical Chemistry
سال: 2009
ISSN: 0165-9936
DOI: 10.1016/j.trac.2008.12.006